Fig. 1.

Effect of LCAT and CETP inhibition on LTIP movement to LDL in plasma. Plasma (750 µl) was preincubated overnight at 4°C with or without anti-CETP monoclonal antibody (27.5 µg). This blocked CETP activity by ∼70%. These treated samples, ± 1 mM paraoxon to block LCAT activity, were subsequently incubated at 37°C for 24 h to induce LTIP movement. Control samples were held at 4°C. A 500 µl aliquot of each sample was then fractionated by FPLC gel filtration (see Methods) and eluted fractions were assayed in triplicate for LTIP by immunoassay. A and B show the LTIP elution profiles. The recovery of LTIP in 37°C incubated samples was 92–105% of 4°C controls. A also shows the distribution of cholesterol in control plasma. The elution position of lipoproteins was not altered by these incubation conditions. Data are representative of three similar experiments.