TABLE 1.

LTIP association with LDL in incubated plasma

Activity Present			LTIP mass (µg)		Plasma LTIP associated with LDL (%)
CETP	LCAT	Temp.	LDL	470 kDa
+	+	4°C	5.7 ± 1.1	29.3 ± 2.9	16.3 ± 1.3
+	+	37°C	22.5 ± 1.7	10.2 ± 0.8	68.7 ± 5.2a
+	–	37°C	12.9 ± 1.0	19.4 ± 1.5	40.0 ± 3.0a
–	+	37°C	17.1 ± 1.3	16.7 ± 1.3	50.6 ± 3.8a
–	–	37°C	4.9 ± 0.4	31.8 ± 2.4	13.3 ± 1.0

After incubation of plasma for 24 h at 4°C or 37°C as described in Fig. 1, the distribution of LTIP among LDL and 470 kDa fractions isolated by FPLC was measured by immunoassay. Negative symbols indicate that CETP or LCAT activity was inhibited by blocking antibody or paraoxon, respectively. Plus symbols designate that the indicated activity was present at endogenous levels. Standard deviations reflect the accuracy of LTIP determinations, which were measured in triplicate. Data are representative of 3 similar experiments.

^aP < 0.01, compared with native plasma at 37°C.