TABLE 3.

Composition of LDL isolated from incubated whole plasma

Active factor	FC	CE	TG	PL	FC+PL/protein	CE+TG/protein	FC+PL/CE+TG
	µg/µg protein
None	0.29 ± 0.01	0.72 ± 0.02	0.15 ± 0.01	0.84 ± 0.01	1.13 ± 0.03	0.87 ± 0.02	1.29 ± 0.04
CETP	0.30 ± 0.01	0.65 ± 0.01	0.41 ± 0.01 a	0.82 ± 0.03	1.12 ± 0.03	1.06 ± 0.02 a	1.06 ± 0.03 b
LCAT	0.19 ± 0.01 a	0.74 ± 0.02	0.16 ± 0.01	0.74 ± 0.02 b	0.93 ± 0.02 b	0.91 ± 0.02	1.03 ± 0.02 b
CETP, LCAT	0.20 ± 0.01 a	0.71 ± 0.01	0.37 ± 0.01 a	0.70 ± 0.01 a	0.90 ± 0.02 a	1.07 ± 0.02 a	0.84 ± 0.02 a

Plasma was incubated (37°C) for 16 h in the presence or absence of 1 mM paraoxon to inhibit LCAT and/or TP2 (46 µg/ml plasma) to inhibit CETP activity. LDL was isolated by sequential ultracentrifugation and chemically characterized as described in the Methods. Based on protein, LDL recovery was 98–111%. Values are the mean ± SD of duplicate determinations. Results are representative of 3 similar experiments.

^aP < 0.01 or

^bP < 0.05 versus control (CETP and LCAT inactive). FC, free cholesterol; CE, cholesteryl ester; TG, triglyceride; PL, phospholipid.