Figure 2.

Photomicrographs of ECM localizations performed on whole cells grown in monolayer culture: For these studies, cells from the same subject were plated at the same density in 6 wells of a multi-well chamber slide and cultured for 10 days. Immunolocalizations were performed for each ECM product using a separate well. Prominent deposits of chondroitin sulfate (Fig. 2A) and keratan sulfate (Fig. 2B) can be seen surrounding the cells. In contrast, localizations for Type I collagen (Fig. 2C) and Type II collagen (Fig. 2D) show no deposition of these collagens by monolayer cultured cells. Figure 2E was taken from a well used as the negative control with deletion of primary antibody for these localizations with hematoxylin staining. (All photomicrographs, X 295).