Fig.4.

Influence of method of HDL isolation on measurements of HDL oxidative activity. Influence of method of HDL isolation on DHR oxidation was assessed using 100 µl in a 384-well flat-bottom plate, and the rate of change in fluorescence was measured as in Fig. 1. The HDL was isolated from 10 healthy volunteers (non-CAD). An amount of 15 µM DHR was exposed to 2.5 µg (cholesterol) of FPLC-purified (FPLC), ultracentrifugation-purified HDL (UC), or HDL that was isolated using dextran sulfate or polyethylene glycol as described in “Materials and Methods.” Rates of oxidation of DHR are plotted as means of quadruplicates for each sample.