Cytochrome c (Cyt-C) release from mitochondria in MLE-12 cells subjected to control, HO-cyclic mechanical stretch (CS), and NAC + HO-CS (1 mM). Cytoplasmic and mitochondrial fractions were separated for Western blotting. Top: cytoplasmic fractions are shown of control (lane 1), NAC + control (lane 2), HO-CS (lane 3), and NAC + HO-CS (lane 4); mitochondrial fractions are shown of control (lane 5), NAC + control (lane 6), HO-CS (lane 7), and NAC + HO-CS (lane 8). Bottom: GAPDH was the loading control for cytoplasmic fractions (lanes 1–4), and cytochrome-c oxidase subunit IV (COX IV) was the loading control for mitochondrial fractions (lanes 5–8). n = 3 Independent experiments in which 4 wells were pooled for each condition. Cytochrome c release was observed in HO-CS, whereas NAC + HO-CS completely blocked cytochrome c release from mitochondria.