Table 4.

Relative expression of select genes measured by quantitative real-time RT-PCR in SC, FF, and HF mice

	Group			P Value
Gene	SC	FF	HF	SC vs. FF	FF vs. HF	SC vs. HF
	Fibrosis
TGFβ1	2.81 ± 0.20	13.42 ± 1.46	4.79 ± 0.18	0.01	0.0009	0.123
ASMA	8.74 ± 3.63	35.12 ± 10.39	16.59 ± 9.22	0.017	0.16	0.38
Collagen type 1α1	0.20 ± 0.05	3.41 ± 0.63	0.69 ± 0.09	0.013	0.014	0.14
Lumican	6.33 ± 0.68	60.47 ± 5.38	10.63 ± 0.57	0.001	0.013	0.11
TIMP1	0.06 ± 0.01	1.97 ± 0.01	0.18 ± 0.03	0.0002	0.0002	0.21
HGF	1.27 ± 0.13	2.52 ± 0.10	1.47 ± 0.05	0.017	0.0066	0.61
Sp1	3.55 ± 0.75	4.52 ± 0.55	3.61 ± 0.16	0.27	0.045	0.93
	Inflammation
TNFα	0.02 ± 0.003	0.20 ± 0.012	0.04 ± 0.004	0.0001	0.0001	0.18
Osteopontin	5.83 ± 1.66	32.16 ± 9.26	5.41 ± 1.00	0.0017	0.0013	0.82
	Fatty Acid trafficking
FABP	6.95 ± 1.20	4.31 ± 0.69	9.52 ± 0.77	0.096	0.0001	0.075
	Cellular stress
SOD1	1.69 ± 0.27	1.17 ± 0.11	1.73 ± 0.12	0.14	0.0032	0.89
CHOP	1.15 ± 0.53	2.86 ± 0.16	1.06 ± 0.06	0.0023	0.0014	0.72
PERK	33.05 ± 0.20	7.04 ± 0.32	3.43 ± 0.10	0.0015	0.0008	0.57
BBC3/PUMA	4.05 ± 1.47	3.68 ± 0.24	2.87 ± 0.27	0.67	0.44	0.22
ATF6	3.98 ± 0.81	5.09 ± 0.49	3.70 ± 0.29	0.28	0.025	0.75
BiP/GRP78	1.38 ± 0.38	1.54 ± 0.19	1.48 ± 0.15	0.73	0.81	0.81

TGFβ1, transforming growth factor-β1; ASMA, anti-smooth muscle actin; TIMP1, tissue inhibitor of metalloproteinase; HGF, hepatocyte growth factor; Sp1, specificity protein 1; FABP, fatty acid-binding protein; CHOP, CCAAT enhancer-binding protein homologous protein; PERK, eukaryotic translation initiation factor 2α kinase 3; PUMA, Bcl-2 binding component 3; ATF6, activating transcription factor 6.