Table 2.
qRT-PCR primer sequences
| Gene Symbol | Direction | Sequence (5′ to 3′) |
|---|---|---|
| 18S | FOR | TCCAAGGAAGGCAGCAGGC |
| 18S | REV | TACCTGGTTGATCCTGCCA |
| Atp7a | FOR | TGAACAGTCATCACCTTCATCGTC |
| Atp7a | REV | GCGATCAAGCCACACAGTTCA |
| Atp7b | FOR | TTAGCATCCTGGGCATGACTTG |
| Atp7b | REV | TTGGTGTGTGAGGAGTCCTCTAGTGT |
| Ctr1 | FOR | CTACTTTGGCTTTAAGAATGTGGACC |
| Ctr1 | REV | AACATTGCTAGTAAAAACACTGCCAC |
| Cp | FOR | ACTTATGCAGATCCTGTGTGCCTATC |
| Cp | REV | TGCATCTTGTTGGACTCCTGAAAG |
| Hamp | FOR | GCAAGATGGCACTAAGCACTC |
| Hamp | REV | GCAACAGAGACCACAGGAGGAAT |
| Heph | FOR | ACACTCTACAGCTTCAGGGCATGA |
| Heph | REV | CTGTCAGGGCAATAATCCCATTCT |
| Mt1a | FOR | CTTCTTGTCGCTTACACCGTTG |
| Mt1a | REV | CAGCAGCACTGTTCGTCACTTC |
| Tfrc | FOR | ATTGCGGACTGAGGAGGTGC |
| Tfrc | REV | CCATCATTCTCAGTTGTACAAGGGAG |
FOR, forward; REV, reverse; Atp7a, Menkes copper transporting ATPase; Atp7b, Wilson's copper transporting ATPase; Cp, ceruloplasmin; Ctr1, copper transporter 1; Hamp, hepcidin; Heph, hephaestin; Mt1a, metallothionein 1A; Tfrc, transferrin receptor 1.