Fig. 1.

Targeted inactivation of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in the intestinal epithelium in PTEN^Lox/Lox/villin^Cre mice. A: wild-type (WT) and PTEN^Lox/Lox/villin^Cre mice were killed at 4 mo of age, and tissue was harvested from various organs. The deleted allele was detected by PCR only in DNA extracted from intestinal epithelial cells isolated from PTEN^Lox/Lox/villin^Cre animals. B-D: confirmation of PTEN deletion in the intestinal epithelium by immunohistochemistry (B), Western blot (C), and qPCR (D). E: PTEN deletion increases phosphorylated (p) protein kinase B (AKT) expression in cells located in the proliferative crypt compartment.