Fig. 5.

A: frequency of adenoma formation in PTEN^WT and PTEN^Lox/Lox/villin^Cre mice at 12 mo of age. B: macroscopic image of a cecal tumor in a PTEN^Lox/Lox/villin^Cre mouse. C and D: H&E staining demonstrating a tubulovillous adenoma (C) and an adenocarcinoma (D) derived from PTEN^Lox/Lox/villin^Cre mice. E and F: tumors derived from PTEN^Lox/Lox/villin^Cre mice show evidence of nuclear β-catenin. Tumors from PTEN^Lox/Lox/villin^Cre mice were stained with β-catenin as indicated in methods. Nuclear β-catenin is indicated by arrows in F. G: negative control. Nonspecific staining is due to the use of an anti-mouse secondary antibody. Shown for comparison in H is nuclear β-catenin in an adenoma derived from an Apc¹⁶³⁸ mouse that harbors an inactivating mutation at codon 1638 of the Apc gene. I and K: pAKT staining in adenomas derived from PTEN^Lox/Lox/villin^Cre and Apc¹⁶³⁸ mice, respectively. J and L: PTEN staining in adenomas derived from PTEN^Lox/Lox/villin^Cre and Apc¹⁶³⁸ mice, respectively. J: PTEN staining in stromal tissue shows specificity of PTEN deletion to epithelial cells.