Fig. 3.

Combination of honokiol and IR induces cell death. A: cell cycle profiles of HCT116 and HCT116-CH3 cells treated with 25 μM honokiol and subjected to 2.5 Gy IR. After 48 h, cells were analyzed by flow cytometry using propidium iodide for DNA content. Honokiol treatment alone increased G₀/G₁ phase of cell cycle, whereas IR increased cell death of HCT116 cells, which is reduced in HCT116-CH3 cells. B: graphs are representative of data collected from 3 independent experiments after treatment with 25 μM honokiol.