Fig. 5.

Effect of chronic alcohol feeding on level of expression of THTR-1 and THTR-2 proteins in rat primary pancreatic acinar cells. Western blot analysis was done using pancreatic acinar whole cell proteins (60 μg) isolated from chronic alcohol-fed rats (4 wk) and their pair-fed controls and were resolved on 4–12% SDS-PAGE and electroblotted onto polyvinylidene difluoride membrane as described in methods. A: blots were incubated with rabbit polyclonal THTR-1 antibody. B: blots were incubated with rabbit polyclonal THTR-2 antibody. Data are means ± SE from 3 different sets of samples from multiple rats and were normalized relative to β-actin protein expression. *P < 0.02, **P < 0.01.