Fig. 8.

Effect of chronic alcohol feeding on level of expression of thiamin pyrophosphokinase (TPKase), thiamin pyrophosphatase (TPPase), and mitochondrial thiamin pyrophosphate (TPP) transporter (SLC19A25) mRNA in rat primary pancreatic acinar cells. Real-time PCR was performed using rat TPKase (A), TPPase (B), and mitochondrial TPP transporter (C) gene-specific primers (see Table 1). Data are means ± SE from 3 separate sets of samples from multiple rats (normalized relative to β-actin) and are calculated by the relative relationship method supplied by the manufacturer (Bio-Rad). *P < 0.05, **P < 0.01.