Fig. 2.

a, b GCs cultivated in medium with FF were viable for up to the ninth passage, demonstrated lower DT and a higher number of PDs. In contrast, GCs cultured in the absence of FF rapidly increased DT, slightly increased the number of PDs (cumulative data), cell died after the third passage. c Telomere length measurement (y-axis;%) in long-term growing cells (GC8 line) and GC-derived tumor cell line COV434. The GC8 line showed a significant decrease in the relative telomere length (y-axis;%) with each increasing passage number (P < 0.01). d Changes in doubling time (DT) versus relative telomere length (ddCt) were significant (P < 0.0001)