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Steady-state kinetic analyses of KshAB with CoA thioesters. Shown is the dependence of the initial velocity of O2 consumption on 1,4-BNC-CoA (A) and 4-BNC-CoA (B) concentrations in air-saturated buffer with fitted parameters Km = 17 ± 3 and 6.8 ± 0.1 μm, and Vmax = 0.56 ± 0.04 and 0.146 ± 0.007 μm s−1, respectively. The best fit of the Michaelis-Menten equation to the data using the least squares dynamic weighting options of LEONORA is represented as a solid line.
