TABLE 1.

Apparent steady-state kinetic parameters of KshAB for various steroids

Experiments were performed using 0.1 m potassium phosphate, pH 7.0, at 22 °C. Values reported in parentheses represent S.E.

Substrate	Km	kcat	Kcat/Km
	μm	s−1	m−1s−1
4-ADa	24 (16)	0.07 (0.01)	3,000 (2,000)
ADDa	110 (20)	0.80 (0.05)	7,600 (700)
4-BNC	3 (2)	0.08 (0.01)	30,000 (10,000)
1,4-BNC	70 (10)	0.25 (0.02)	3,500 (400)
4-BNC-CoA	6.8 (0.1)	0.61 (0.03)	90,000 (10,000)
1,4-BNC-CoA	17 (3)	2.7 (0.2)	160,000 (20,000)

^a Values presented in Capyk et al. (15).