FIGURE 5.
PEX5 diaromatic motifs involved in the NDPEX14-induced disruption of the mCat-PEX5 interaction. A, schematic representation of recombinant PEX5 proteins. The diaromatic motifs in the N-terminal half of PEX5 are numbered 1–7. Replacement of tryptophan and phenylalanine/tyrosine residues by alanines in these motifs is indicated by X. B–D, 35S-labeled catalase was synthesized in vitro for 55 min (lane 55′) and chased for 4 h in the absence (lane −) or presence of 1 μm of the indicated recombinant PEX5 proteins alone or together with NDPEX14 (30 μm). Samples were analyzed by native-PAGE/autoradiography.