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. 2011 Sep 23;286(47):40900–40910. doi: 10.1074/jbc.M111.286146

FIGURE 2.

FIGURE 2.

Expression of caveolins in A2780 and A2780/HPR cells. Panel A, semi-quantitative PCR analysis of CAV1, -2, and -3 mRNA expression levels was performed in A2780 and A2780/HPR cells by simultaneous amplification of the housekeeping genes GAPDH and ACTB. Human skeletal muscle satellite cells (SMSC) have been used as positive control for CAV3 expression. Patterns (left) are representative of those obtained in three independent experiments. CAV3 signal was not detectable in A2780 and A2780/HPR cells. Right, in the graphs, data are normalized versus GAPDH expression and utilizing individual CAV1 and -2 of the A2780 specimen set as 1. Data are the means ± S.D. of three independent experiments. *, p < 0.01 versus A2780 cells. Panel B, caveolin-1 protein levels were assessed by Western blotting. Equal amounts of cellular proteins (corresponding to 30 μg) were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes. Membranes were probed using specific anti-caveolin-1 and anti-β-actin monoclonal antibodies. Patterns (left) are representative of those obtained in three independent experiments. Right, in the graphs, the histogram represents mean densitometric quantification of caveolin-1. Data are normalized versus β-actin expression and utilizing caveolin-1 of the A2780 specimen set as 1. Data are the means ± S.D. of three independent experiments. *, p < 0.01 versus A2780 cells.