FIGURE 2.

Transcription start sites of P_nanTp and P_nanE and sequence analysis of the regulatory region of the nan operons. A, TSSs, indicated by asterisks, were determined by primer extension of the RNA derived from the wild type (A₆₀₀ 0.6) grown with LBS. Lanes C, T, A, and G represent the nucleotide sequencing ladders of pBS0909. B, TSS of P_nanTp and P_nanE are indicated by bent arrows, and the positions of their putative −10 and −35 regions are underlined. The NanR- and CRP-binding sequences determined later in this study (Fig. 5) are presented as a shaded box and a dotted box, respectively. The conserved nucleotide sequences for the binding of CRP are indicated above the V. vulnificus DNA sequence in capital letters. The translation initiation codons (ATG in boldface) and coding regions of nanT_P and nanE (open arrows) are also presented.