FIGURE 7.
Effects of Neu5Ac and its catabolic intermediates on the NanR repression of PnanE in vitro. Supercoiled plasmid pBS0921 containing the PnanE was used as a template DNA and transcribed in vitro in the presence of 100 nm NanR. Either Neu5Ac or one of its catabolic intermediates (1 mm) such as ManNAc, GlcNAc, ManNAc-6P, GlcNAc-6P, and GlcN-6P were added to the supercoiled template DNA as indicated. The 370-base PnanE-specific transcripts and vector-derived control transcripts RNA-1 are indicated.