TABLE 1.
Plasmids and bacterial strains used in this study
| Strain or plasmid | Relevant characteristicsa | Ref. or source |
|---|---|---|
| Bacterial strains | ||
| V. vulnificus | ||
| ATCC29307 | Wild type V. vulnificus, virulent | Laboratory collection |
| DI0201 | ATCC29307 with Δcrp | 19 |
| HG072 | ATCC29307 with nanA::nptI, Kmr, Rifr | 13 |
| BS0805 | ATCC29307 with ΔnanR::nptI, Kmr | This study |
| BS0902 | BS0805 with Δcrp, Kmr | This study |
| BS1012 | HG072 with ΔnanR, Kmr, Rifr | This study |
| BS1104 | ATCC29307 with ΔnanE | This study |
| E. coli | ||
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λ pir; Kmr; host for π-requiring plasmids; conjugal donor | 18 |
| BL21 (DE3) | F−, ompT, hsdS (rB−, mB−), gal (DE3) | Laboratory collection |
| Plasmids | ||
| pGEM-T easy | PCR product cloning vector; Apr | Promega |
| pBS0909 | pGEM-T easy with intergenic region between nanTP and nanE; Apr | This study |
| pDM4 | Suicide vector; oriR6K; Cmr | 17 |
| pBS1013 | pDM4 with ΔnanR; Cmr | This study |
| pBS0907 | pDM4 with Δcrp ; Cmr | This study |
| pBS1102 | pDM4 with ΔnanE ; Cmr | This study |
| pHIS-Parallel1 | Protein expression vector; Apr | 22 |
| pBS0820 | pHIS-Parallel1 with nanR; Apr | This study |
| pHK0201 | pRSET A with crp; Apr | 23 |
| pSH0505 | pET28a with V. vulnificus rpoD, Kmr | 25 |
| pRLG770 | General transcription vector; Apr | 27 |
| pBS0921 | pRLG770 with PnanE; Apr | This study |
a The following abbreviations are used: Apr, ampicillin-resistant; Cmr, chloramphenicol-resistant; Kmr, kanamycin-resistant; Rifr, rifampicin-resistant.