FIGURE 7.

Coculture induced apoptosis resistance in NCM460 cells depends on Nrf2 and proteasomal gene expression. NCM460 cells were transfected with (A) Nrf1 or Nrf2 and (B) α5 or S5a siRNAs and submitted 24 h later to either mono- or coculture (M-g) for 3 days. Then cells were treated with 20 μg/ml irinotecan or 10 ng/ml TRAIL for 24 and 8 h, respectively. The cells were submitted to a caspase-3/7 assay for the determination of apoptosis. All of the data represent the means ± S.D. of five independent experiments. *, p < 0.05 compared with the corresponding monocultured cells.