FIGURE 6.
shRNA-mediated knockdown of β1 integrins reduces CaSR-stimulated cell migration. Cells were treated with one of three shRNA constructs as follows: a scrambled control and two β1 integrins shRNAs (#1 and #2). A, Western blot probed with anti-β1 integrin antibody and anti-GAPDH antibody (loading control). B, effects of shRNA on cell adhesion (1 μg/ml fibronectin); C, effects of shRNA on cell migration (10 μg/ml fibronectin). B and C, each column is the average ± S.E. of 2–3 experiments. *, p < 0.05.