TABLE 2.
Transient-state kinetic constants of AAO and three Phe-501 variants for p-methoxybenzyl alcohol and O2 substrates
Transient-state kinetic constants were determined in 0.1 m phosphate (pH 6) at 12 °C. Rate constants were fitted to Equations 3 and 4 for reductive and oxidative half-reactions, respectively. Means ± S.D. are provided.
| Reductive half-reaction |
Oxidative half-reaction |
||
|---|---|---|---|
| kred | Kd | kox(app) | |
| s−1 | μm | s−1mm−1 | |
| AAO | 139 ± 16 | 26 ± 5 | 657 ± 30 |
| F501Y | 87 ± 1 | 15 ± 1 | 401 ± 9 |
| F501W | 136 ± 6 | 362 ± 45 | 1524 ± 1 |
| F501A | 35 ± 5 | 153 ± 2 | 8 ± 1 |