TABLE 1.
Differences in Cys sensitivity and IC50 of various SATs
Data represent the average of three replicate protein samples with three independent assays each. IC50 represents the Cys concentration that inhibits 50% of the SAT activity.
| Isoforms | Inhibition by l-Cys | IC50 |
|---|---|---|
| μm | ||
| AtSAT-c | Sensitive | 3.3a |
| TgSAT-m | Insensitive | >100b |
| TgSAT-p | Insensitive | >100b |
| TgSAT-c | Insensitive | >100b |
| Tg-N/At-C | Sensitive | 3.4a |
| At-N/Tg-C | Insensitive | >100b |
| C268P | Sensitive | 83.9c |
| G270A | Sensitive | 17.7d |
| C268P/G270A | Insensitive | >100b |
a,b,c,d Values followed by different letters within a treatment are significantly different (one-way analysis of variance, p < 0.05, n = 9).