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. 2011 Oct 10;286(47):40575–40583. doi: 10.1074/jbc.M111.274910

FIGURE 1.

FIGURE 1.

Reduced bone formation and decreased osteoblast differentiation in Fgf2−/− female mice compared with wild-type littermates. Femoral BMD (A) and lumber vertebrae BMD (B) were decreased significantly in Fgf2−/− mice. Reduced osteoblast mineralization (C), reduced osteocalcin (D), and Col1a1 (E) mRNA expression in Fgf2−/− compared with Fgf2+/+ BMSCs are shown. (Black line represents Fgf2+/+, and gray line represents Fgf2−/−). A and B, BMD was determined in 6-month-old female Fgf2+/+ (n = 7) and Fgf2−/− (n = 8) mice. C, freshly isolated BMSCs were cultured in α-MEM/10% FBS for 9 days, then in α-MEM/10% FBS with 50 μg/μl ascorbic acid and 8 mm β-glycerophosphate for another 8 days, followed by alkaline phosphatase, von Kossa staining, and quantification of total mineralization area. Data are mean ± S.E. of four independent experiments using 8-month-old male mice. D and E, freshly isolated BMSCs from 12-month-old female mice were cultured in α-MEM/10% FBS for 3 days, then in α-MEM/10% FBS with 50 μg/μl ascorbic acid and 8 mm β-gly-cerophosphate until day 17, followed by total RNA extraction for gene analysis. Similar results were also obtained in 8- and 10-month-old female mice.