FIGURE 7.

A, representative plot of RT-(primer-template) complex binding stability in the presence of Mg²⁺, Mn²⁺, and Zn²⁺. Half-lives are indicated with their respective curves. Results are an average of 3 exp. ± standard deviations. B, representative plot of bound RT (nm, determined by extension) versus [HIV-RT] (nm) used to determine the K_d values in the presence of Zn²⁺ or Mg²⁺. Experiments were performed as described under “Experimental Procedures.”