FIGURE 2.
HSPC038 overexpression increases IClswell. IClswell activation was monitored in HEK293 Phoenix cells overexpressing both HSPC038 and the transfection marker EGFP as separate proteins. Experiments were done in the whole cell configuration. a, original recordings obtained in hypertonic (upper traces) or hypotonic conditions (lower traces) in HSPC038 transfected (HSPC038-IRES-EGFP) or control (EGFP-IRES-EGFP) cells with voltage increments of 20 mV from −120 to +100 mV applied at a holding potential of 0 mV. b and c, the current density-to-voltage (b) and the current density-to-time relationships (c) showing an up-regulation of IClswell. The data were fitted with second order polynomials following application of the extra sum of squares F test (EGFP-IRES-EGFP versus HSPC038-IRES-EGFP in hypertonicity: not significant; in hypotonicity: p < 0.0001).