FIGURE 4.

Purified ICln and HSPC038 increase ICl_swell. ICl_swell activation was monitored in HEK293 Phoenix cells in the whole cell patch clamp configuration. The current density-to voltage relationship (15 min after hypotonic shock) (a) and the respective current density-to-time graph (b) of ICl_swell were measured with 1.76 μm of purified ICln alone or with an equimolar (1.76 μm each) amount of both ICln and HSPC038 proteins in the pipette filling solution. The data were fitted with second order polynomials following application of the extra sum of squares F test (ICln and HSPC038 versus ICln alone: p < 0.0001).