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. 2011 Sep 14;286(47):40659–40670. doi: 10.1074/jbc.M111.260430

FIGURE 5.

FIGURE 5.

Molecular interaction between ICln and HSPC038. For each panel, HSPC038, ICln and its truncation mutants or a mix of HSPC038 and ICln or its truncation mutants separated by native PAGE are shown in the first, second, and third lanes, respectively. A band of higher molecular mass (asterisk), indicating the presence of a complex between ICln and HSPC038, was detected for full-length ICln (ICln 1–235, n = 5) (a), ICln 1–159 (n = 5) (b), and ICln 1–134 (n = 4) (c), but not for ICln 158–235 (n = 2) (d). Affinity chromatography eluates separated by SDS-PAGE of ICln 1–235 (e), ICln 1–159 (f), ICln 1–134 (g), and ICln 158–235 (h). For each panel, first lane, input; second lane, elution; and third lane, control (i.e. the elution fraction from beads with no HSPC038 bound to the resin). The presence of a band in the elution fraction indicates that full-length ICln or its truncation mutants interacted with HSPC038. No interaction was detected for ICln 158–235 (h); 2 ≤ n ≤ 4.