FIGURE 6.
Specific infectivity of prions from RML- or 22L-infected cells is not affected by propagation in swa. PK1 cells were infected with 10−3 diluted RML- or 22L-infected brain homogenate and propagated for five 1:10 splits, about 25 doublings, to yield PK1[RML]wp-P3 and PK1[22L]wp-P3. The populations were then grown for 7 days in the absence or presence of 2 μg of swa/ml. Cell lysates were adjusted to contain equal concentrations of rPrPSc, as determined by Western blotting. PK1 cells were infected with a serial dilution of lysates of PK1[RML]wp-P3 cells (A) or PK1[22L]wp-P3 cells (B), treated with swa (red) or untreated (blue) and subjected to the SSCA. The number of rPrPSc-positive cells per 20,000 cells is plotted against dilution of lysate. There was no difference in the infectivity of complex-glycosylated rPrPSc and swa-misglycosylated rPrPSc.