FIGURE 7.
Incorporation of 35S-labeled amino acids into PrPSc in presence or absence of swa. On day 0, 106 PK1 cells (A) or LD9 cells (B) in 10-cm dishes were infected with 10−4 diluted RML-infected brain homogenate. On day 4, the medium was changed (PK1) or the cells split at 1:5 ratio (LD9) into medium with or without 2 μg/ml swa. On day 8, the cells were pulsed with 35S-labeled methionine and cysteine for 2 h, and the label was chased for 4 and 6 h. rPrPSc was purified by immunoprecipitation, and recovery of rPrPSc was assessed by Western blotting, and deglycosylated radioactive rPrPSc was assessed by autoradiography. The graphs show 35S-rPrPSc corrected for recovery. AR, autoradiogram, WB, Western blot. The experiment in A was performed in duplicate and that in B in singlicate.