FIGURE 7.

Effect of Leptopilina recombinant SOD3 and venom on Drosophila PO activity. PO activity was measured as absorbance at 490 nm for A, D. melanogaster larval hemolymph in presence of buffer alone, Trx-LbmSOD3 (3–0.003 μg), Trx-tag alone (3 μg), or PO inhibitor (PTU). B, D. melanogaster larval hemolymph in the presence of buffer alone, 3 μg of Trx-LbmSOD3, Trx-LhSOD3, BtSOD or Trx tag alone, or PTU. C, D. melanogaster larval hemolymph in presence of Trx-LhSOD3 (3 μg), Trx-tag alone (3 μg), or PTU added either before or after PO activation. Mean values (±S.E.) are given for each category (three replicates). Different letters above the bars designate significantly different values.