TABLE 1.

Predicted hsa-miR-138 targeting sites on FOLS1 mRNA

The accession number for the FOSL1 mRNA sequences used in this analysis is NM_005438.3.

Positiona	Location	Adjacent siteb	Seed seq. c	Typed	Predicted mfee
174	5′-UTR		CgCCAGC	Non-canonical	−25.1 kcal/mol
742	CDS	Yes	uACCAGu	Non-canonical	−23.7 kcal/mol
751	CDS	Yes	CACCAGC	Canonical	−26.3 kcal/mol
762	CDS	Yes	CACCAGC	Canonical	−25.4 kcal/mol
1424	3′-UTR	Yes	CACCAGC	Canonical	−22.6 kcal/mol
1436	3′-UTR	Yes	ugCCAGC	Non-canonical	−21.8 kcal/mol

^a The bp location of the start of the seed sequence in the FOSL1 mRNA sequence.

^b If the distance between the start positions of two seed sequences is less than 23 bp (the length of miR-138 molecule), then these targeting sites are defined as adjacent sites.

^c The seed sequences (seq.) are identified from FOSL1 mRNA as 7-mers that are reverse complementary to the sequence from the 2 to 8 positions of hsa-miR-138. The canonical bases are identified by uppercase letters, and the non-canonical bases are identified by lowercase letters.

^d The canonical targeting site is defined by the presence of seed sequence CACCAGC in the mRNA molecule. The non-canonical targeting site allows G:U wobble base pairing. The seed sequence on the mRNA molecule allows the substitution of C by U and the substitution of A by G.

^e The minimum free energy (mfe) for the binding of hsa-miR-138 to the targeting sequence in FOSL1 mRNA is predicted using the RNAhybrid program (32). An mfe cut-off of −20.0 kcal/mol was used.