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HeLa cells CHMP2B protrusions do not contain actin or tubulin. Immunostaining with anti-β-tubulin antibody (A) and staining with Texas Red (Tx Red)-phalloidin (B) of HeLa cells overexpressing CHMP2B demonstrate the absence of tubulin and F-actin in CHMP2B tubes. C, latrunculin A treatment (0.1 μg/ml; 60 min) induces depolymerization of actin but does not collapse CHMP2B tubes.
