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Phosphorylation of MCM3 affects its loading onto chromatin. A, 293T cells were transfected with indicated plasmids. Total cell lysates and chromatin-bound fraction were prepared and subjected to immunoblot with indicated antibodies. β-Actin and lamin B were used as the loading controls. B, quantitcation of chromatin-bound MCM3 and its mutants. The wide-type MCM3 was arbitrarily set to 100. Data are represented as mean ± S.D. (n = 3).
