FIGURE 3.

Confirmation of C203S-CypD mutation by restriction digestion and DNA sequencing. In A, 1 μg of mutated CypD (mCypD) plasmid was digested with 2 units of restriction enzyme, NotI, at 37 °C for 1 h. The resulting reaction and undigested samples were analyzed on a 1% agarose gel. A representative ethidium bromide stained gel is shown. In B, a mutated C203S-CypD plasmid sample was sequenced to confirm the site-directed mutagenesis. The replaced serine corresponding codon TCT is in boldface.