FIGURE 5.

mPTP opening is blocked by C203S-CypD. CypD^−/− MEFs (KO) were transfected either with WT CypD or C203S-CypD plasmids for 48 h as labeled in each panel. After transfections, mPTP opening was assessed using H₂O₂ (A and B) or ionomycin (C) in the presence and absence of an NO donor, GSNO, or CsA using the calcein-cobalt quenching technique. In D, after transfections, CRC was measured using calcium-sensitive probe calcium green-5N in the presence of 5 μm Ca²⁺ pulses. A representative figure is shown from three independent assays. *, p < 0.05 versus WT+empty or KO+CypD (n = 5). mCypD, mutant CypD.