FIGURE 7.

Induction of mutated C203S-CypD expression in the liver mitochondria of CypD^−/− mice abolished Ca²⁺-induced mPTP opening. Liver samples were obtained 72 h after adenovirus injections with an adenoviral vector driving WT CypD, mutated C203S-CypD, or AdCMV-GFP (used as a control) expressions. Immunoblot analysis of 40 μg of mitochondrial protein extracted from mouse livers, and the resulting blots were probed with anti-CypD, GFP, and β-ATP synthase (ATPsyn) antibodies. Representative blots are shown (n = 6). mCypD, mutant CypD.