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. 2011 Sep 12;286(46):40060–40068. doi: 10.1074/jbc.M111.275255

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — PK resistance of H2H3 and H2H3Flag. A, spontaneous PK resistance of H2H3 and H2H3Flag. Cell lysates from 100% confluent and 100% confluent + 2 days cultures were PK-digested. D3, F6, and B5 displayed PK-resistant proteins, in contrast to E1 cells. B, comparative PK resistance assay between Chandler-infected SN56 cells and overconfluent F6 cells. Increasing concentrations of PK (μg/mg of total proteins) were used, and samples were analyzed by Western blot using Saf83 antibody for ScSN56 and M2 for F6 cells. Negative controls (Neg) included uninfected SN56 cells and 100% confluent F6 cells. C, bright field observation of E1, D3, F6, and B5 cells at 100% confluence and 100% confluence + 2 days (2d), with a 10× objective. Only E1 cells at 100% confluence + 2 days did not show visible refringent dead cells.