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In vivo interaction of WT UCE and R328C-UCE. HeLa cells in 100-mm dishes were transiently transfected with 20 μg of WT UCE-FLAG and 10 μg of R328C-UCE-HA (lanes 1–3) or transfected with 20 μg of WT UCE-FLAG only (lanes 4–6) or 10 μg of R328C-UCE-HA only (lanes 7–9). After 24 h, cell lysates were prepared and subjected to immunoprecipitation (IP) with anti-FLAG Ab or anti-HA Ab. The precipitates were subjected to SDS-PAGE and Western blotting with both antibodies.
