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. 2011 Sep 28;286(46):39786–39793. doi: 10.1074/jbc.M111.295899

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Effect of R328C-UCE on maturation of WT UCE. HeLa cells in 100-mm dishes were transiently transfected with 7 μg of WT UCE-FLAG and 7 μg of R328C-UCE-HA (A, lanes 1, 2, 5, and 6) or transfected with 7 μg of WT UCE-FLAG only (A, lanes 3 and 4) or 7 μg of R328C-UCE-HA only (B, lanes 7 and 8). After 24 h, cell lysates were prepared, treated with or without Endo Hf, and immunoprecipitated (IP) with anti-FLAG Ab or anti-HA Ab. The precipitates were subjected to SDS-PAGE and Western blotting (IB) using both antibodies.