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KLF5 colocalizes with SMURF2. COS-1 cells were transfected with HA-KLF5 and Myc-SMURF2, treated with MG132 to stabilize HA-KLF5, and stained with chicken anti-HA and rabbit anti-Myc, followed by FITC-conjugated donkey α-chicken and Cy5-conjugated donkey α-rabbit secondary antibodies. A, representative staining of three different cells, including two with both nuclear and cytoplasmic KLF5. B, representative staining of multiple cells in the same field.
