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. 2011 Sep 21;286(46):39933–39944. doi: 10.1074/jbc.M111.241620

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — VEGF-A signaling via VEGFR2-nephrin induces cell shape change. A, COS-7 cells transfected with FLAG-VEGFR2, Myc-nephrin, or both constructs (Ne+VR2) were exposed to vehicle or rVEGF₁₆₅ for 30 min, fixed, and stained with rhodamine-phalloidin. VEGF₁₆₅ induced cell shape change only in expressing nephrin+VEGFR2 cells. B, morphometric analysis of cell area showed a decrease in cell size in VEGFR2-expressing cells (yellow bar), and a more pronounced decrease in cell size in nephrin+VEGFR2-expressing cells (red bar). Cell area (μm²) expressed as mean ± S.E., 15 + 2.4 cells/experimental condition. Asterisks indicate p < 0.05 versus vehicle; #, p < 0.05 Ne+VR2 versus VEGFR2 alone. C, FLAG and Myc WB from COS-7 cells transfected with VEGFR2 (lane 1), nephrin (lane 2), and nephrin+VEGFR2 (lane 3), actin is shown as loading control.