The depolarization enhances the interaction between PKC βII and PI4 kinase β. A, co-immunoprecipitation results for PKC α or PKC βII interaction. Protein lysates from oocytes treated with ND96, ND96-K, or PMA (50 nm) were immunoprecipitated and blotted with anti-PI4 kinase β, anti-PKC α, or anti-PKC βII antibodies. B, summary data for the relative intensity of the precipitates blotted with anti-PI4 kinase β, anti-PKC α, or anti-PKC βII antibodies from five independent experiments. *, p < 0.05 compared with the control.