FIGURE 6.

Response of PRC-dependent inflammation/stress genes to diverse metabolic insults. A, a dominant negative allele of NRF-1 (pSG5/dnNRF-1) was expressed in U2OS cells, and the level of PRC protein relative to that in cells transfected with the empty vector control (pSG5) was measured by immunoblotting (insert). Sp1, endogenous NRF-1, and dnNRF-1 were assayed as controls. The expression of the subset of seven inflammation/stress genes was compared with that of the respiratory genes described in the legend to Fig. 2 by quantitative real time PCR (filled bars). Results were normalized to the values obtained from the pSG5-transfected control. B, U2OS cells were plated and after 24 h were fed with glucose-containing (+) or glucose-depleted (−) medium. After 16 h PRC protein was assayed by immunoblotting (insert). The expression of the subset of seven inflammation/stress genes was compared with that of the respiratory genes described in the legend to Fig. 2 by quantitative real time PCR. Results were normalized to the values obtained from the glucose-fed cells. For both panels, values are the averages ± S.E. for at least three separate determinations.