TABLE 1.
Effect of OPN on the number of osteoclasts exhibiting oscillations in [Ca2+]i
Rat osteoclasts were plated on coverslips coated with BSA or OPN and loaded with fura-2 as described under “Experimental Procedures.” Individual osteoclasts were monitored for periods of at least 25 min. Data show the number of osteoclasts exhibiting oscillations in [Ca2+]i, which were defined as a series of at least 2 transient elevations in the 345/380 ratio of amplitude at least 2 times that of the maximum level of baseline fluctuations. A greater proportion of osteoclasts exhibited oscillations when plated on OPN compared to BSA (significantly different based on chi-square test, p < 0.05). Data are based on responses from a total of 25–26 osteoclasts for each condition from 6 independent preparations of cells.
Substrate | Oscillations | No oscillations | Proportion of osteoclasts exhibiting oscillations |
---|---|---|---|
BSA | 7 | 18 | 28% |
OPN | 16 | 10 | 62% |