FIGURE 7.

Distribution of NMN deamidase and other NAD biosynthetic enzymes in bacteria. The phylogenetic tree of the representative set of 100 bacterial genomes was taken from the Genomic Encyclopedia of Bacteria and Archaea project (43). Genomic identifiers (locus tags) of genes encoding PncC proteins are listed in the first column. The color code reflects domain composition and enzymatic activity as in Fig. 5. Proteins experimentally characterized in this work are marked by a red star. Distribution of NAD biosynthetic enzymes in bacterial genomes was taken from the “NAD and NADP cofactor biosynthesis global” subsystem in the SEED genomic database. Functional roles of these enzymes are described in Fig. 1.