FIGURE 3.

A gle1–4 mutant displays genetic interactions with DED1. A–C, cultures of indicated single and double mutant strains with wild-type controls (WT) were 5-fold serially diluted and spotted on YPD, then incubated 2 days at 25 °C, 27.5, 30, or 37 °C. Strains included cold-sensitive ded1–120 (A–C), gle1–4 (A), ipk1Δ (B), and rat7-1 (nup159) (C). D, wild-type and gle1–4 strains transformed with galactose-inducible DED1 (pGAL-DED1), DED1-R438G (pGAL-DED1-R/G), or control plasmids were incubated at the indicated temperatures for 3 days on selective media containing galactose (top) or glucose (bottom).