FIGURE 6.

Aβ-induced neuronal loss is prevented by inhibition of phagocytosis. Mixed neuronal/glial cultures were treated for 3 days with Aβ1–42 (250 nm) with or without phagocytosis inhibitors. A, cytochalasin D (cytoD, 1 μm, added at 48 h of treatment); B, annexin V (AnxV, 100 nm, added with Aβ1–42); C, phosphatidylserine antibody (α-PS) or mouse control IgG (5 μg/ml, added with Aβ1–42); D, annexin V (AnxV, 100 nm) and phosphatidylserine antibody (α-PS) added at 48 h of treatment; E, cyclo(RGDfV) peptide (cRGD) or control peptide cyclo(RADfV) (cRAD, 50 μm, added with Aβ1–42). F and G, cultures were treated with Aβ1–42 and cRGD for 7 days. Data are presented as means ± S.E. for ≥3 independent experiments. ***, p < 0.001 versus control; ###, p < 0.001 versus Aβ1–42. Scale bars, 25 μm.